Mitochondria-Associated Gene SLC25A32 as a Novel Prognostic and Immunotherapy Biomarker: From Pan-Cancer Multiomics Analysis to Breast Cancer Validation

Figure 9

SLC25A32 promotes the proliferation, invasion, and migration of breast cancer. (a) Quantitative RT-PCR results comparing SLC25A32 expression levels in healthy breast epithelium and four breast cancer cell lines. (b) The 231 and 549 cell lines were selected for subsequent experiments, and SLC25A32 expression levels were verified using western blotting after knocking down both fragments. The blots were cropped from the full-length original blot to improve the clarity and conciseness of the presentation. (c) Grey value ratio of target/reference protein in 231 and 549 cell lines. (d, e) Changes in SLC25A32 mRNA levels post-transfection in 231 and 549 cells were verified using PCR. (f, g) CCK-8 assay was used to detect the proliferation of 231 and 549 cells post-transfection. Plate cloning assay was used to detect the colony-forming ability of the two cell lines post-transfection. (h, i) Plate cloning assay was used to detect the colony-forming ability of the two cell lines post-transfection. ; .

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