FAP Serves as a Prognostic Biomarker in Head and Neck Squamous Cell Carcinoma

Figure 3

Regulatory role of FAP in cellular proliferation and apoptosis in HNSCC. (a) Western blot analysis across diverse HNSCC cell lines unveils elevated FAP expression in the SCC15 cell line. (b) Following FAP knockout in SCC15 cells, WB data clearly reveal a notable downregulation of FAP expression. (c) qPCR assays provide further evidence of the efficiency of FAP silencing in SCC15 cells. (d) A significant decline in SCC15 cell proliferation was observed post-FAP knockout, as confirmed by CCK8 assays. (e and f) Apoptotic status of SCC15 cells post-FAP knockout: (e) depicts a representative micrograph of apoptotic cells, and (f) offers a quantitative assessment of apoptosis. (g) WB data confirm a marked increase in FAP expression following its overexpression in HN6 cells. (h) qPCR assays validate the efficiency of FAP overexpression in HN6 cells. (i) FAP overexpression led to a noticeable enhancement in the proliferation of HN6 cells, as evidenced by CCK8 assays. (j and k) Apoptotic status of HN6 cells post-FAP overexpression: (j) shows a typical image of apoptotic cells, and (k) presents a quantitative analysis of apoptosis.   and .

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