Analytical Validation of TaqMan™ Assays on the OpenArray™ Platform Using Applied Biosystems QuantStudio™ 12K Flex for the Rapid Identification of Nail Microbiota

Table 2

The validation experiment strategy.

OpenArray	Day	NTC	Synthetic control	gDNA	Exclusivity	Human gDNA	Extracted	Total	Comment
1	1	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
2	1	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
3	1	0	0	0	0	0	24	24	Plasmid
4	2	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
5	2	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
6	2	0	0	0	0	0	24	24	Plasmid
7	3	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
8	3	6	1	11	1	1	4	24	Positive controls, specificity controls, plasmid, organism pools, NTC
9	3	0	0	0	0	0	24	24	Plasmid
10	Any one day	3	18	0	0	0	0	21	Plasmid dilutions for linear range