Analytical Validation of TaqMan™ Assays on the OpenArray™ Platform Using Applied Biosystems QuantStudio™ 12K Flex for the Rapid Identification of Nail Microbiota

Table 6

The assay specificity of the nail panel was detecerrted at 99.90% across all assays and controls tested.

Assay ID	Assay	Correct	Correct	Wrong	Wrong
APFVRD9	A. alternata	96	100.00%		0.00%
APAACG3	A. fumigatus	90	100.00%		0.00%
AP9HMYA	A. israelii	96	100.00%		0.00%
AICSXMN	A. niger	90	100.00%		0.00%
APCFAW4	A. strictum	96	100.00%		0.00%
APKA736	A. tenuissima	96	100.00%		0.00%
Fn07921946_s1	Alternaria spp.	96	100.00%		0.00%
Fn04646233_s1	C. albicans	90	100.00%		0.00%
APYMNR3	C. jeikeium	96	100.00%		0.00%
Fn07921952_s1	C. lunata	96	100.00%		0.00%
APAAAT7	C. neoformans	87	96.67%	3	3.33%
Fn04646221_s1	C. parapsilosis	90	100.00%		0.00%
Fn04646220_s1	C. tropicalis	90	100.00%		0.00%
Fn07921937_s1	E. floccosum	96	100.00%		0.00%
Fn07921967_s1	F. solani	96	100.00%		0.00%
Fn07921976_s1	G. candidum	96	100.00%		0.00%
APCE439	M. furfur	96	100.00%		0.00%
APPRN34	M. guilliermondii	96	100.00%		0.00%
Fn07921982_s1	M. gypseum	90	100.00%		0.00%
Ba04230908_s1	mecA	90	100.00%		0.00%
AP329KC	N. dimidiatum	96	100.00%		0.00%
AP7DTKZ	Nocardia	96	100.00%		0.00%
Ba04932081_s1	P. aeruginosa	90	100.00%		0.00%
Ba04646259_s1	S. aureus	90	100.00%		0.00%
APMF3XK	S. brevicaulis	96	100.00%		0.00%
AP47W2P	S. hyalinum	96	100.00%		0.00%
Fn07921951_s1	T. interdigitale	90	100.00%		0.00%
AP9HN97	T. mentagrophytes	90	100.00%		0.00%
AP326G9	T. mucoides	96	100.00%		0.00%
Fn07921933_s1	T. rubrum	90	100.00%		0.00%
Fn07921974_s1	T. tonsurans	96	100.00%		0.00%
APDJY9N	T. verrucosum	96	100.00%		0.00%
Ac00010014_a1	Xeno	84	100.00%		0.00%
	Grand total	3075	99.90%	3	0.10%