Advanced Gut & Microbiome Research

Research Article

Analytical Validation of TaqMan™ Assays on the OpenArray™ Platform Using Applied Biosystems QuantStudio™ 12K Flex for the Rapid Identification of Nail Microbiota

Table 7

The overall reproducibility, which included day-to-day as well as operator-to-operator variation, was 99.91% across all assays tested.
Assay IDTarget organismTotal concordantTotal discordantReproducibility
APFVRD9A. alternata102100.00%
APAACG3A. fumigatus102100.00%
AP9HMYAA. israelii102100.00%
AICSXMNA. niger102100.00%
APCFAW4A. strictum102100.00%
APKA736A. tenuissima102100.00%
Fn07921946_s1Alternaria spp.102100.00%
Fn04646233_s1C. albicans102100.00%
APYMNR3C. jeikeium102100.00%
Fn07921952_s1C. lunata102100.00%
APAAAT7C. neoformans99397.06%
Fn04646221_s1C. parapsilosis102100.00%
Fn04646220_s1C. tropicalis102100.00%
Fn07921937_s1E. floccosum102100.00%
Fn07921967_s1F. solani102100.00%
Fn07921976_s1G. candidum102100.00%
APCE439M. furfur102100.00%
APPRN34M. guilliermondii102100.00%
Fn07921982_s1M. gypseum102100.00%
Ba04230908_s1mecA102100.00%
AP329KCN. dimidiatum102100.00%
AP7DTKZNocardia102100.00%
Ba04932081_s1P. aeruginosa102100.00%
Ba04646259_s1S. aureus102100.00%
APMF3XKS. brevicaulis102100.00%
AP47W2PS. hyalinum102100.00%
Fn07921951_s1T. interdigitale102100.00%
AP9HN97T. mentagrophytes102100.00%
AP326G9T. mucoides102100.00%
Fn07921933_s1T. rubrum102100.00%
Fn07921974_s1T. tonsurans102100.00%
APDJY9NT. verrucosum102100.00%
Ac00010014_a1Xeno102100.00%
99.91%