Analytical Validation of TaqMan™ Assays on the OpenArray™ Platform Using Applied Biosystems QuantStudio™ 12K Flex for the Rapid Identification of Nail Microbiota

Table 8

The LOD for the assays was 10,000-20,000 copies input to extraction.

Assay ID	Assay	cp input into extraction	cp/μl (20 ul input)	cp/throughout (60 μl elution vol.)	Ct average
APFVRD9	A. alternata	10000	500	3	28.87
APAACG3	A. fumigatus	10000	500	3	30.27
AP9HMYA	A. israelii	20000	1000	6	29.78
AICSXMN	A. niger	10000	500	3	29.14
APCFAW4	A. strictum	100000	5000	28	28.36
APKA736	A. tenuissima	10000	500	3	29.92
Fn07921946_s1	Alternaria spp.	10000	500	3	30.01
Fn04646233_s1	C. albicans	10000	500	3	28.63
APYMNR3	C. jeikeium	10000	500	3	30.61
Fn07921952_s1	C. lunata	20000	1000	6	29.21
APAAAT7	C. neoformans	10000	500	3	29.66
Fn04646221_s1	C. parapsilosis	10000	500	3	28.78
Fn04646220_s1	C. tropicalis	10000	500	3	28.87
Fn07921937_s1	E. floccosum	20000	1000	6	28.66
Fn07921967_s1	F. solani	10000	500	3	29.34
Fn07921976_s1	G. candidum	10000	500	3	28.91
APCE439	M. furfur	10000	500	3	29.46
APPRN34	M. guilliermondii	30000	1500	8	28.07
Fn07921982_s1	M. gypseum	10000	500	3	30.31
Ba04230908_s1	mecA	20000	1000	6	27.16
AP329KC	N. dimidiatum	10000	500	3	27.21
AP7DTKZ	Nocardia	10000	500	3	29.09
Ba04932081_s1	P. aeruginosa	10000	500	3	29.29
Ba04646259_s1	S. aureus	10000	500	3	28.81
APMF3XK	S. brevicaulis	20000	1000	6	29.29
AP47W2P	S. hyalinum	10000	500	3	26.57
Fn07921951_s1	T. interdigitale	10000	500	3	30.11
AP9HN97	T. mentagrophytes	10000	500	3	28.26
AP326G9	T. mucoides	20000	1000	6	29.57
Fn07921933_s1	T. rubrum	10000	500	3	30.16
Fn07921974_s1	T. tonsurans	20000	1000	6	29.52
APDJY9N	T. verrucosum	10000	500	3	29.98
Ac00010014_a1	Xeno	10000	500	3	23.47