Research Article

Cohort Profile: A Prospective Study of Gut Microbiota in Patients with Acute Ischemic Stroke

Table 1

Summary of variables collected for the Wenzhou ischemic stroke cohort study.

ClassificationContentsMethod

General dataName, gender, age, occupation, place of residence, contact information, education level, marital status, family history, etc.Questionnaire/interview
Medical historyPast history and current medicationQuestionnaire/interview
Health-related behaviorsSmoking and drinking historyQuestionnaire/interview
Anthropometric measuresHeight and weightExamination
Blood pressureSystolic blood pressure and diastolic blood pressureExamination
Biochemical indicators
Urine and common itemsProteins, ketones, blood, bilirubin, and nitratesExamination
Blood and common itemsCBC, total protein, albumin, FPG, triglyceride, total cholesterol, HDL-cholesterol, BUN, creatinine, uric acid, AST, ALT, hs-CRP, HbA1c, B12, folic acid, and HcyExamination
Blood and additional itemsLipid markers (LDL-cholesterol), hemostatic markers (prothrombin time, activated partial thromboplastin time, fibrinogen, D-dimer), cardiac marker (troponin I), and thyroid function markers (FT3, FT4, TT3, TT4 and TSH)Examination
Imaging itemsMagnetic resonance imaging (MRI), CT angiography (CTA) or magnetic resonance angiography (MRA), cervical vessels ultrasonography and transcranial Doppler), heart rhythm (ECG and 24-Holter monitoring), and cardiac morphology (transthoracic echocardiogram).Examination
StoolPCR on 16 s rRNA gene sequence.
PCR on 18 s rRNA gene sequence.
Laboratory analysis
BloodGC-MS was used to detect the metabolites of gut microbiotaLaboratory analysis

CBC: complete blood count; FPG: fasting plasma glucose; HDL-cholesterol: high density lipoprotein cholesterol; BUN: blood urea nitrogen; AST: aspartate aminotransferase; ALT: alanine aminotransferase; hs-CRP: high sensitivity C-reactive protein; HbA1c: hemoglobin A1c, B12, vitamin B12; Hcy: homocysteine; LDL-cholesterol: low density lipoprotein cholesterol; FT3: free triiodothyronine; FT4: free tetraiodothyronine; TT3: total triiodothyronine; TT4: total thyroxine; TSH: thyroid-stimulating hormone; GC-MS: gas chromatography-mass spectrometry.