Cohort Profile: A Prospective Study of Gut Microbiota in Patients with Acute Ischemic Stroke

Table 2

Contents and scoring rules of cohort follow up.

Objective examination	Follow-up project	Major diagnostic sections
Neurologic disability measures	mRS	0—No symptoms.; 1—No significant disability. Able to carry out all usual activities, despite some symptoms.; 2—Slight disability. Able to look after own affairs without assistance but unable to carry out all previous activities.; 3—Moderate disability. Requires some help but able to walk unassisted.; 4—Moderately severe disability. Unable to attend to own bodily needs without assistance and unable to walk unassisted.; 5—Severe disability. Requires constant nursing care and attention, bedridden, and incontinent.; 6 - dead
Poststroke insomnia (PSI)	PSQI	(1) Subjective sleep quality; (2) Sleep latency; (3) Sleep duration; (4) Habitual sleep efficiency; (5) Sleep disturbances; (6) Use of sleep medications; (7) Daytime dysfunction
	ICSD-3	(1) Insomnia; (2) Sleep-related breathing disorders; (3) Central disorders of hypersomnolence; (4) Circadian rhythm sleep-wake disorders; (5) Parasomnias; (6) Sleep-related movement disorders; (7) Other sleep disorders
Posstroke depression (PSD)	HAMA	(1) Psychic anxiety; (2) Somatic anxiety
	HAMD	7 subscales: anxiety/somatization, weight cognitive impairment, diurnal variation, blockage, sleep disorders, and hopelessness
Dietary habits	Diet questionnaire	(1) What is the proportion of grains, potatoes, beans, fish, meat, oil, fruits, and vegetables in your diet?; (2) What kind of staple food do you eat? Rice or noodles?; (3) What is your usual taste? Sweet or salty? Light or fried?; (4) Have you used antibiotics or probiotics in the last month?; (5) Do you exercise regularly? What kind and frequency of exercise?

mRS: modified Rankin’s scale; PSQI: Pittsburgh’s sleep quality index; ICSD-3: international classification of sleep disorders-third edition; HAMA: Hamilton’s anxiety scale; HAMD: Hamilton’s depression.