Research Article

Proteomic Characterization of Human Gut Habitual Bacteroides intestinalis against Common Intestinal Bile Acid Stress

Table 1

B. intestinalis DEP response to BA toxicity and differential expression between TDCA and TLCA.

IDProtein nameToxicity correlationTDCA/TLCA ratioFunction

B3C6N7Outer membrane efflux protein (tolC)+2.38Efflux pump
B3C7S6Arginine-tRNA ligase (ArgRS)+2.28Protein synthesis
B3C7S7DNA topoisomerase 1+2.12Transcription
B3C860Transcription termination/antitermination protein (NusG)+2.08Transcription
B3CIX4Zinc metalloprotease (rseP)+2.05Protease
B3CIN3Histidine-tRNA ligase (HisRS)+2.01Protein synthesis
B3CAL5Tetratricopeptide repeat protein+1.98Unknown
B3CFC8Peptidase, S41 family+1.95Protein synthesis
B3C535Trigger factor (tig)+1.93Protein folding
B3CCX7Antioxidant, AhpC/TSA family (PCB)+1.92Antioxidant
B3CE86Peptidyl-prolyl cis-trans isomerase D (ppiD)+1.77Protein folding
B3C5L3Hydrolase, NUDIX family+1.76Nucleotide metabolism
B3CEA730S ribosomal protein S1+1.75Protein synthesis
B3C6P750S ribosomal protein L19+1.64Protein synthesis
B3C865DNA-directed RNA polymerase subunit beta (rpoB)+1.58Transcription
B3C7Y8Phosphate butyryltransferase (ptb)--1.56SCFA production
B3C5T4Methylmalonyl-CoA mutase (MUT)--1.58SCFA production
B3CJ01Glucose-1-phosphate thymidylyltransferase--1.60Nucleotide metabolism
B3CGR4Dihydroorotase (URA4)--1.62Nucleotide metabolism
B3CED1Methionine aminopeptidase (MetAP, map)--1.68Protein synthesis
B3CB15Pyridine nucleotide-disulfide oxidoreductase--1.68Energy metabolism
B3CGC4Glucosamine-6-phosphate deaminase-like protein (nagB)--1.70Energy metabolism
B3CEC7OmpA family protein--1.77Biofilm formation
B3CBG5Hydroxylamine reductase (HCP) (prismane protein)--1.80Nitrate metabolism
B3CII2Transcriptional regulator, effector binding domain protein (AraC)--1.83Transcription
B3C6Z9Acetate kinase (acetokinase, ackA)--1.85SCFA production
B3CF224-Phosphoerythronate dehydrogenase (hprA)--1.87Energy metabolism
B3CH61Uridylate kinase (UMPK, pyrH)--1.88Nucleotide metabolism
B3CGH3Alpha-2-macroglobulin family protein--1.89Host colonization
B3C7F4Peptidase dimerization domain protein (argE), acetylornithine deacetylase--1.92Amino acid/polyamine metabolism
B3C5N1Polyprenyl synthetase geranylgeranyl diphosphate synthase (GGPS)--1.94Diterpene synthesis
B3C707Cupin family protein--1.99Unknown
B3C9Z3Fructokinase, PfkB family--2.01Energy metabolism
B3CFW4Orotidine 5-phosphate decarboxylase (OMPdecase, pyrF)--2.03Nucleotide metabolism
B3C5D8DJ-1 family protein, protein deglycase (thiJ)--2.06Redox sensor
B3CF616-Phosphogluconolactonase (PGLS)--2.09Energy metabolism
B3CD72Aspartate carbamoyltransferase regulatory chain (pyrl)--2.09Nucleotide metabolism
B3C86350S ribosomal protein L10--2.12Protein synthesis
B3C8L7Carbamoyl-phosphate synthase small chain (carA)--2.17Amino acid metabolism

Note: positively (+) and negatively (-) correlated with BA toxicity.