Variations in Gut Microbiome Profiles Based on Different DNA Extraction Methods: A Comparative Study

Figure 1

Workflow of experimental design. Four DNA extraction methods (CTAB, QIAamp, TIANGEN, and MAGEN) were compared for frozen faecal samples from five mammals (human, macaque, dog, golden hamster, and mouse). The bacterial DNA extracted using these methods was tested for concentration, purity, and integrity and then subjected to 16S rRNA gene sequencing for microbial community analysis. Finally, a certain community of defined microbiota was introduced to mimic the complex composition of the gut microbiota in vivo and to validate the efficacy of the optimal DNA extraction method.