Asn¹² and Asn²⁷⁸: Critical Residues for In Vitro Biological Activity of Reteplase

Figure 4

SDS-PAGE analysis of Pichia derived native rPA and its mutants. M: Medium protein molecular weight marker (97 to 14 kDa); lane 1: native rPA (∼55 kDa); lane 2: (N12P mutant ∼43 kDa); lane 4: (N12P, N278S mutant, ∼39 kDa); lane 4: (N278S, ∼43 kDa) lane 5: GS115 control.