Asn¹² and Asn²⁷⁸: Critical Residues for In Vitro Biological Activity of Reteplase

Table 1

Oligos used for carrying out SDM of wild type (native) rPA gene.

Asn^12	FP: 5^′ GGC AAC AGC GAT TGC TAT TTT GGC CCC GGG AGC GCG TAT CGC GGC ACC 3^′
	RP: 5^′ GGT GCC GCG ATA CGC GCT CCC GGG GCC AAA ATA GCA ATC GCT GTT GCC 3^′
Asn^278	FP: 5^′ GC ACA TCA CAA CAT TTA CTT TCT AGA ACA GTC ACC GAC AAC 3^′
	RP: 5^′ GTT GTC GGT GAC TGT TCT AGA AAG TAA ATG TTG TGA TGT GC 3^′