DNMT3A Mutations in Patients with Acute Myeloid Leukemia in South Brazil

Table 2

Primer sequences for genes of interest.

Chromosomal translocation	Fusion transcript	Sequence (5′–3′)
t(8;21)	AML1-A	CTACCGCAGCCATGAAGAACC
	ETO-B	AGAGGAAGGCCCATTGCTGAA
	PML-A1	CAGTGTACGCCTTCTCCATCA
t(15;17)	PML-A2	CTGCTGGAGGCTGTGGAC
	RAR𝛼-B	GCTTGTAGATGCGGGGTAGA
inv16	CBF𝛽-A	GCAGGCAAGGTATATTTGAAGG
	MYH11-B2	TCCTCTTCTCCTCATTCTGCTC
t(9;11)	MLL6S	GCAAACAGAAAAAAGTGGCTCCCCG
	AF9AS3	TCACGATCTGCTGCAGAATGTGTCT
Gene	Exon	Sequence (5′–3′)
DNMT3A	Exon 19	CACCACTGTCCTATGCAGACA
		ATTAGTGAGCTGGCCAAACC
DNMT3A	Exon 20	CCTTGGCTCATCTTCAAACC
		CACTATGGGTCATCCCACCT
DNMT3A	Exon 21	CCGCTGTTATCCAGGTTTCT
		CCCAGCAGAGGTTCTAGACG
DNMT3A	Exon 22	TTTGGTAGACGCATGACCAG
		AGCACAGCAATCAGAACAGC
DNMT3A	Exon 23	TCCTGCTGTGTGGTTAGACG
		ATGATGTCCAACCCTTTTCG