In-the-Field Authentication of Grapevine (Vitis vinifera L.) cv: Albariño Using Chlorotype Discrimination and a Single SNP Interrogation by LAMP

Figure 5

Colorimetric LAMP reactions (12.5 μL) performed on standard PCR tubes in a portable thermoblock using in-field DNA isolations following the Li17 method. (a) The template DNA obtained directly from four lysates (2 replicates per lysate) is tested with the reference REF system. (b) The DNA from one lysate (3 replicates) is tested with the ctA (Chl-A) and ctD (Chl-D) systems to identify the carried chlorotype. Reactions included a positive (C+) and negative control (NTC).

(a)

(b)