Australian Journal of Grape and Wine Research

Research Article

In-the-Field Authentication of Grapevine (Vitis vinifera L.) cv: Albariño Using Chlorotype Discrimination and a Single SNP Interrogation by LAMP

Table 1

Oligonucleotide sequences for LAMP interrogation of chlorotypes for cultivar authentication in Vitis vinifera L. FIP and BIP oligos included F1c-F2 and B1c-B2 sequences, respectively.
Oligo nameSequence 5′⟶3′
Reference LAMP oligo system
Vvin-ref-loc7.1.F3GGA​TTT​TAT​AGC​AAA​TGC​CAG​T
Vvin-ref-loc7.1.B3AGT​TCC​ACA​TGA​GAA​GGT​G
Vvin-ref-loc7.1.FIPCTG​CTG​TGG​GAA​TCT​TTT​AAG​CAC-TGA​TAC​TTT​CTT​CAC​GCA​CT
Vvin-ref-loc7.1.BIPCTT​TCA​TCG​CAT​ATT​ACA​CTC​TCC​A-TTT​GTT​CGA​GCT​GCC​ATC
Vvin-ref-loc7.1.LFCCT​CCC​CAA​TCT​CAC​CCT​ATC
Vvin-ref-loc7.1.LBAAT​CCA​CGA​GAT​CCT​TCA​CTG​A
Chlorotype A specific LAMP oligo system
Vvin-ctA + 54.1.F3CGG​TCT​TGA​ATT​ATA​TTG​GAT​AGC​T
Vvin-ctA + 54.1.B3TCC​CAT​TTT​ATC​TAA​TCC​TTA​TTG​TC
Vvin-ctA + 54.1.FIPGCA​CTC​AGA​AAC​TCT​CAT​GAA​TCT​GT-GGG​GGA​ATC​TAA​TTA​GTA​GTT​GT
Vvin-ctA + 54.1.BIPAAT​TCT​TTC​TTT​TCG​GTA​ATC​CCT​AGT​C-CCC​GAT​TGT​TTC​ACA​GAG​A
Vvin-ctA + 54.1.LFATA​CAA​AGT​ATC​TTC​TGC​CCT​TTC​C
Vvin-ctA + 54.1.LBAAC​GAT​AGA​CTG​TCT​CCC​GAT​T
Chlorotype D-specific LAMP oligo system
Vvin-ctD − 54.4.F3TTA​GTA​GTT​GTG​GAA​AGG​GCA
Vvin-ctD − 54.4.B3GGG​AAT​GTT​ACT​AAT​GAA​CAT​GTA​AG
Vvin-ctD − 54.4.FIPCCG​AAA​GAA​AGA​ATT​TCT​TTT​TGC​ACT​T-ACT​TTG​TAT​ACA​GAT​TCA​TGA​GAG​TT
Vvin-ctD − 54.4.BIPAAT​TCT​TTC​TTT​CGG​GAG​ACA​ATA​AGG​A-GTT​CCC​AGT​ATT​AAT​GAA​AGG​CA
Vvin-ctD − 54.4.LFATT​GAT​TGA​ATG​CTC​GAG​CAC​TCA​G
Vvin-ctD − 54.4.LBGGG​AAA​TAG​AGG​TAT​GTG​ATA​GTA​GAT​AGT​GAT