Research Article

In-the-Field Authentication of Grapevine (Vitis vinifera L.) cv: Albariño Using Chlorotype Discrimination and a Single SNP Interrogation by LAMP

Table 2

Design of oligos for LAMP interrogation of the diagnostic SNP 191_100 aimed at the identification of the “Albariño” variety. FIP and BIP oligos included F1c-F2 and B1c-B2 sequences, respectively.

Oligo nameSequence 5′⟶3′

Vvin-191.2.F3TTCAAACAGAAAACARAAGGA
Vvin-191.1.B3TGAAACTGTGACCCCAAT
Vvin-191.C.1.FIPGGA​AAG​TTT​TTA​TTT​TCC​CTT​TGG​GA-ACT​TGT​GAT​GTT​CAC​AGA​TGA
Vvin-191.C.1.BIPCAG​CCA​AAC​CAA​GAT​GCT​TTG-CTT​CAA​ATG​GGG​CAC​TAC​A
Vvin-191.T.1.FIPAGA​AAG​TTT​TTA​TTT​TCC​CTT​TGG​GA-ACT​TGT​GAT​GTT​CAC​AGA​TGA
Vvin-191.T.2.BIPTAG​CCA​AAC​CAA​GAT​GCT​TTG​TC-AAA​TGG​GGC​GCA​CTA​CA
Vvin-191.1.LFAAT​CTA​TAG​GGA​TTA​ACT​ACT​TTT​CAA​ATC​T
Vvin-191.2.LBGTCCAGCAATTMGGTTCTYGAATG