A 3D Osteoblast In Vitro Model for the Evaluation of Biomedical Materials

Table 1

Parameters and scores for the qualitative assessment of the spheroids.

	Score
	1	2	3	4
Regularity of shape	Mostly amorphous	Mostly irregular	Mostly regular spheroids	Completely spheroidal
Size	Too small	Small	Medium	Big
Repeatability	Strong variation in shape, size, and number	Variation in one or two parameters	Most parameters are similar	Very similar in all parameters
Handling	Very difficult	Difficult	Easy	Very easy
Stability	Always disintegrate	Disintegrate on a considerable proportion (after 2-3 days)	Most remain stable	All remain stable after 2-3 days