A Turbidity Test Based Centrifugal Microfluidics Diagnostic System for Simultaneous Detection of HBV, HCV, and CMV

Table 1

Reagent values for each virus.

	Unit	HBV	HCV	CMV
Betaine	M	1	0.8	0.8
MgSO4	mM	4	6	10
dNTPs	mM	1.6	1.4	1.4
Bst polymerase	U	8
F3/B3	μM	0.2
LF/LB	μM	0.4	1	0.8
FIP/BIP	μM	1.6	2	1.6
Temperature	°C	63	65	65
Time	Minutes	45	60	60
DNA volume	μL	5
Sensitivity	Copies	50	60	10