Comparison of the Effect of Extracted Bacteriocin and Lytic Bacteriophage on the Expression of Biofilm Associated Genes in Streptococcus mutans

Table 1

Oligonucleotide sequences of primers and PCR amplification program.

Target gene	Primer sequence (5′-3′)	PCR product (bp)	PCR programs	Ref
gtfD	F:_GGCACCACAACATTGGGAAGCTCAGTT-R:_GGAATGGCCGCTAAGTCAACAGGAT-	431	1 cycle: 95°C (5 min); 35 cycle: 94°C (45 s), 59°C–68°C (based on primers, 60 s), 72°C (60 s); 1 cycle: 72°C (5 min)	[10]
gtfB	F:-AGCAATGCAGCCAATCTACAAAT-R:_ ACGAACTTTGCCGTTATTGTCA-	96		[11]
gtfC	F:_ CTCAACCAACCGCCACTGTT-R:_GGTTTAACGTCAAAATTAGCTGTATTAG-	91		[11]