Research Article
Global Analysis in Nonobstructive Azoospermic Testis Identifies miRNAs Critical to Spermatogenesis
Table 1
Primer sequences for qRT-PCR of selected miRNAs and U6.
| | miRNA ID | Primer | Sequence |
| | miR-449a-5p (MIMAT0001541) | RT | 5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCAGC-3 | | F | 5-AGGCGTGGCAGTGTATTGTT-3 |
| | miR-888-5p (MIMAT0004916) | RT | 5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGACTG-3 | | F | 5-GGCGCGTACTCAAAAAGCTG-3 |
| | U6 (gene ID: 26827) | RT | 5-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAATATG-3 | | F | 5-ATGACGCAAATTCGTGAAGC-3 | | R | 5-CAGTGCAGGGTCCGAGGTA-3 |
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Abbreviation: RT: reverse transcription; F: forward; R: universal reverse.
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