Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

Figure 4

Histological changes and oxidative stress in the testes induced by the Mel and CP treatments. (a) The seminiferous tubules of CP-treated mice showed vacuolization and atrophy compared with the CN and Mel groups, which was restored by Mel cotreatment with the normal cellular content in seminiferous tubules. SP: spermatogonia; PS: pachytene spermatocytes; RS: round spermatids; ES: elongated spermatids. showed vacuolization in the seminiferous epithelium. The vacuolization in the seminiferous epithelium was shown as pentagonal plum blossom, not hexagonal plum blossom. (b) The diameter of the seminiferous tubule, the thickness of the seminiferous epithelium, and the number of Leydig cells in interstitial space were analyzed by HE photographs. (c) Western blot of oxidative stress proteins in testes. ; .

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