Research Article

Melatonin Ameliorates Cyclophosphamide-Induced Spermatogenesis Disorder by Reducing Pyroptosis

Figure 5

Mel promoted the proliferation and meiosis of germ cells which were damaged by CP. (a) IHC was carried out against PCNA to visualize spermatogenic cells. PCNA was mainly found in the nucleus of spermatogonia. The scale bar represented 50 μm. (b) The PCNA-positive cells were statistically analyzed under ten fields of each group. (c) Western blotting was carried out against proliferation-related protein PCNA and meiosis-related proteins REC8, STRA8, and SYCP3. (d) The relative integrated density of the bands of PCNA, REC8, STRA8, and SYCP3. Results were expressed as , , and .
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