Yangjing Capsule Increases Testosterone Production through SET/PI3K/Akt Pathway in Leydig Cell

Figure 1

YC-medicated serum upregulates SET expression and promotes steroidogenesis in MLTC-1 cells. MLTC-1 cells were treated with 10% control serum (CS), low (LS), medium (MS), and high (HS) doses of YC-medicated serum, respectively for 48 hr. The testosterone levels of the culture medium in each group were determined by ELISA (a). SET, StAR, P450scc, and HSD17B mRNA and protein expression levels were determined by real-time PCR and Western blot, respectively (b), (c) and (d). .

(a)

(b)

(c)

(d)