Yangjing Capsule Increases Testosterone Production through SET/PI3K/Akt Pathway in Leydig Cell

Figure 3

SET knockdown blocks YC-medicated serum-induced testosterone production and upregulated steroidogenic enzymes in MLTC-1 cells. MLTC-1 cells were divided into four groups, control serum (CS) group, high-dose YC-medicated serum (HS) group, CS + SET knockdown (CS + si-SET) group, and HS + SET knockdown (HS + si-SET) group. Testosterone levels in culture medium (a), mRNA expression (b), and protein expression (c) and (d) of SET, StAR, P450scc, and HSD17B in MLTC-1 cells were detected by ELISA, real-time RT-PCR, and Western blot, respectively. .

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