Yangjing Capsule Increases Testosterone Production through SET/PI3K/Akt Pathway in Leydig Cell

Figure 6

YC extract reduces apoptosis of Leydig cells in mice. The elder mice (aged 18 months) were randomly divided into four groups including old control, low dose (0.63 g/kg YC), medium dose (1.26 g/kg YC), and high dose (2.52 g/kg YC). Young mice aged 12 weeks were allocated in the sham group. TUNEL staining was used to assess cell apoptosis in testis sections of each group (a) and (b). Protein expression levels of Akt, P-Akt, Bcl-2, Bax, and cleaved-caspase-3 in testes of each group were assessed by Western blot (c) and (d). The mechanism of YC regulation of PI3K/Akt signaling pathway to reduce Leydig cell apoptosis (e). .

(a)

(b)

(c)

(d)

(e)