ALKBH5 Inhibits Cancer Cell Proliferation in Prostate Cancer through KLF4/TERT Signaling

<div>ALKBH5 downregulates the expression levels of KLF4 mRNA by reducing its stability. (a) After addition to 50 <i>μ</i>g/mL CHX or 10 <i>μ</i>M MG132 or DMSO, the impact of ALKBH5 on KLF4 protein expression was detected by western blotting. (b) Luciferase reporter was performed to verify whether ALKBH5 binds to KLF4 gene. (c, d) ALKBH5 silencing increased the stability and half-life of KLF4 mRNA. (e, f) YTHDF1 silencing decreased the stability and half-life of KLF4 mRNA. (g) RIP-qPCR was performed to verify interaction between YTHDF1 protein and KLF4 transcripts. 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Andrologia

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Figure 4

Figure 4: ALKBH5 Inhibits Cancer Cell Proliferation in Prostate Cancer through KLF4/TERT Signaling 