ALKBH5 Inhibits Cancer Cell Proliferation in Prostate Cancer through KLF4/TERT Signaling

Figure 5

KLF4 is a critical downstream target of ALKBH5 to suppress PCa growth. (a) The expression of KLF4 in clinical PCa tissues was analyzed by IHC. (b) IRS of KLF4 in PCa tissues. (c) Correlations between KLF4 and ALKBH5 expression (). (d–g) Following the transfection of LNCaP-shALKBH5 cells with siRNA targeting KLF4, the expression of KLF4, TERT, and CD44 was detected by western blotting (d). The effect of KLF4 on PCa cell proliferation ability was measured by MTS assay (e). Plate clonality assays were used to measure cell clonality (f). Q-TRAP assay was used to evaluate telomerase activity (g). .

(a)

(b)

(c)

(d)

(e)

(f)

(g)