Research Article

Outcomes of Microdissection Testicular Sperm Extraction/Intracytoplasmic Sperm Injection in Cases of Nonobstructive Azoospermia: A Retrospective Study

Table 5

Fertilization and pregnancy outcomes of ICSI for NOA using fresh versus thawed sperm collected by microdissection testicular sperm extraction.

VariableFresh spermThawed sperm value

Number of cycles5645
Female age29 (26–31)28 (26–32.5)0.784
Infertility duration (years)3 (2–6)3 (1.5–5.5)0.796
Follicles punctured ()17.5 (13–22.75)16 (12–22.5)0.475
Total oocytes ()14 (10–19)13 (11–17.5)0.959
MII oocytes ()11 (8.25–15)11 (9–15)0.921
Oocytes used for ICSI ()11 (8–15)11 (9–15)0.875
Zygotes ()8 (6.25–10)6 (3–9)0.007
Fertilization rate (%)74.92% (484/646)52.86% (277/524)<0.001
Two-pronuclear (2PN) zygotes (n)8 (6–9)5 (2.5–8)0.008
2PN fertilization rate (%)68.27% (441/646)48.47% (254/524)<0.001
2PN cleavages ()7 (6–9)5 (2.5–8)0.010
2PN cleavage rate (%)98.87% (436/441)98.82% (251/254)0.955
High-quality embryos ()4 (2–6)2 (1–4)0.007
High-quality embryo rate (%)52.06% (227/436)43.82% (110/251)0.038
Blastocysts formed ()1 (0–4)1 (0–3)0.370
Blastocyst formation rate (%)55.79% (130/233)54.30% (82/151)0.774
Available blastocysts ()1 (0–2)0 (0–2)0.421
Available blastocyst rate (%)73.85% (96/130)70.73% (58/82)0.620

Notes: data are presented as median (interquartile range) or percentage (number ratio); .