Novel NLRP14 Mutations Induce Azoospermia

Figure 1

Identification of the NLRP14 mutation. (a) Conventional end-point PCR was performed to amplify exon 5 of the NLRP14 gene from genomic DNA, and PCR product sequences from the three brothers and their parents were verified by the Sanger sequencing and aligned to human NLRP14 cDNA. (b) Conventional end-point PCR was performed to amplify exon 10 of the NLRP14 gene from genomic DNA, and the PCR product sequences from the patient and his parents were verified by the Sanger sequencing and aligned to human NLRP14 cDNA.

(a)

(b)