The Difference in Subpopulation Structures in Sex-Sorted and Non-Sorted Semen by Discriminant Analysis of Bull Sperm Motility Data Collected by a Computer-Assisted Sperm Analysis System

Figure 2

The structures of sperm motility subpopulations in sex-sorted and non-sorted bull semen at 0, 2, and 4 h after incubation. Three bulls are used and three replicates are performed in each bull. Number of sperm analyzed by the CASA are 1,316 in sex-sorted and 1,578 in non-sorted semen. Asterisks indicate a significant difference in the percentage of motile sperm between sex- and non-sorted sperm (). ^{A, B}Different letters indicate significant differences in the proportion of cluster 1 between incubation times (). ^{a, b}Different letters indicate significant differences in the proportion of cluster 3 between incubation times (). ^{X, Y}Different letters indicate significant differences in cluster 1 between sex- and non-sorted sperm (). ^{x, y}Different letters indicate significant differences in cluster 3 between sex- and non-sorted sperm ().