Ultra-Rapid Freezing and Rapid Freezing Methods in Clinical ICSI Program: Effects on Sperm Biological Characteristics, DNA Methylation Stability, DNA Methyltransferase Activity, and Embryo Morphokinetics

Figure 2

Ultra-rapid freezing method and warming: 1. sperm suspension (0.15); 2. inner straw (0.25 ml); 3. outer straw (0.5 ml); 4. sealed area of straw with heat; and 5. warming medium.