Ultra-Rapid Freezing and Rapid Freezing Methods in Clinical ICSI Program: Effects on Sperm Biological Characteristics, DNA Methylation Stability, DNA Methyltransferase Activity, and Embryo Morphokinetics

Table 3

Spearman’s correlation analysis of DNA methylation and DNMTs activity with DFI and sperm parameters in fresh, ultra-rapid freezing, and rapid groups.

		DNA methylation	DNMTs	DFI	Progressive motility	Viability	Morphology
Fresh group
DNA methylation	Correlation	1	0.657^b	0.029	0.561^b	−0.155	0.401
	p-Value	—	0.011	0.921	0.037	0.596	0.156
DNMTs	Correlation	0.657^b	1	−0.054	0.604^b	0.063	0.096
	p-Value	0.011	—	0.855	0.022	0.832	0.744
Ultra-rapid freezing group
DNA methylation	Correlation	1	0.732^a	−0.247	0.672^a	0.014	−0.082
	p-Value	—	0.003	0.395	0.009	0.962	0.781
DNMTs	Correlation	0.732^a	1	−0.081	0.637^b	−0.261	0.009
	p-Value	0.003	—	0.782	0.014	0.367	0.976
Rapid group
DNA methylation	Correlation	1	0.617^b	0.354	0.629^b	−0.324	0.199
	p-Value	—	0.019	0.214	0.016	0.258	0.495
DNMTs	Correlation	0.617^b	1	0.215	0.724^a	−0.326	−0.077
	p-Value	0.019	—	0.46	0.003	0.255	0.793

^aCorrelation is significant at (two-tailed); ^bcorrelation is significant at (two-tailed).