Ultra-Rapid Freezing and Rapid Freezing Methods in Clinical ICSI Program: Effects on Sperm Biological Characteristics, DNA Methylation Stability, DNA Methyltransferase Activity, and Embryo Morphokinetics

Table 4

Comparison of laboratory characteristics between different groups.

Characteristics	Fresh	Ultra-rapid freezing	Rapid	p-Value
Total number of; MII oocytes (n)	330	330	330	—
Fertilization rate (%), (n/n)	75.5%, (249/330)^a	73.6%, (243/330)^a	72.1%, (238/330)^a	0.62
Cleavage rate (%), (n/n)	90.4%, (225/249)^a	86.8%, (211/243)^a,b	81.5%, (194/238)^b	0.01
Blastocyst rate (%), (n/n)	42.2%, (105/249)^a	34.2%, (83/243)^a,b	30.3%, (72/238)^b	0.02

Note. The used superscript letters that are similar do not show a significant difference (), but the dissimilar letters show a significant difference ().