Research Article
Ultra-Rapid Freezing and Rapid Freezing Methods in Clinical ICSI Program: Effects on Sperm Biological Characteristics, DNA Methylation Stability, DNA Methyltransferase Activity, and Embryo Morphokinetics
Table 5
Comparison of morphokinetics individual and calculated variables between groups.
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Note. tPB2, time to detached the second PB from oolema; tPNa, time to PN appearance; tPNf, time to PN disappearance; t2, first cleavage (2-cell stage); t3, second cleavage (3-cell stage); t4, 4-cell stage; t5, 5-cell stage; t6, 6-cell stage; t7, 7-cell stage; t8, 8-cell stage; tM, time to morula; tB, time to blastocyst; tEB, time to expanded blastocyst; cc2 = t3–t2; s2 = t4–t3; cc3 = t5–t3; s3 = t8–t5. , , ; the different between fresh with ultra-rapid freezing and rapid groups. †; the different between ultra-rapid freezing with rapid groups. |