Research Article

A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

Figure 1

(a, b) Immunohistochemistry (IMH) analysis of Klf4 expression in the seminiferous tubules and comparing it with SOX9: (a1) blue for 4,6-diamidino-2-phenylindole (DAPI) was used to stain cell nuclear; (a2) red fluorescence shows the Klf4 expression pattern, which has a high expression in the central parts of seminiferous tubules that means the expression of Klf4 is upregulated in the adluminal compartment during spermatogenesis; (a3) representation of the merged images (scale bar: 100 μm); (b1) blue shows DAPI; (b2) green fluorescence shows the expression of SOX9, which shows the location of Sertoli cells; (b3) merge image of Sertoli cells marker with DAPI (scale bar: 50 μm).
(a)
(b)