A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

Figure 2

The difference of the protein expression pattern of Klf4 in two populations of spermatogonia by immunocytochemical (ICC) analysis in vitro. Wight arrows indicate undifferentiated SSCs population, and the yellow arrows indicate differentiated SSCs population: (a) the bright field image of two populations of SSCs; (b) blue for 4^′,6-diamidino-2-phenylindole (DAPI) was used to stain cell nuclear; (c) red fluorescence shows the expression pattern of Klf4, which has sharp expression in differentiated cells but a low expression in undifferentiated population of SSCs; (d) representation of the merged images with DAPI (scale bar: 50 μm).

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