A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

Figure 3

(a, b) Comparison of the Klf4 expression pattern in the two cell populations of SSCs: (a1–a3) shows the Klf4 pattern in the undifferentiated population of SSCs, and (b1–b3) shows the Klf4 expression pattern in the differentiated population of SSCs; (a1 and b1) blue for 4^′,6-diamidino-2-phenylindole (DAPI) was used to stain the nucleus of cells; (a2 and b2) red fluorescence indicates the expression pattern of Klf4 that has higher expression in the differentiated population than in the undifferentiated population of SSCs; (a3 and b3) representation of the merged images with DAPI (scale bar: 50 μm for b1–b3 and 100 μm for a1–a3).

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