Research Article

A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

Figure 4

Comparison of expressions of Klf4 and Vimentin in differentiated populations of spermatogonia by immunocytochemical (ICC) analysis in vitro. Arrows indicate differentiated SSCs. The expression pattern of Klf4 and Vimentin in SSCs are the same, and Vimentin is also expressed in MEF cells in culture medium: (a) blue shows DAPI; (b) green fluorescence shows Klf4; (c) red fluorescence shows Vimentin; (d) representation of the merged images (scale bar: 100 μm).
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