Research Article

A Study of Dual Function and Expression Pattern of Klf4 between Two Different Processes of Differentiation and Maintenance of Pluripotency in Spermatogonial Stem Cells

Figure 5

Comparison of expressions of Klf4 and Ki67 in two populations of spermatogonia by immunocytochemical (ICC) analysis in vitro. Yellow arrows indicate differentiated cells and white arrows show undifferentiated SSCs. The expression pattern of Klf4 and Ki67 is the same, and both of them are more expressed in differentiated cells than in undifferentiated cells: (a) blue shows DAPI; (b) green fluorescence shows Ki67; (c) red fluorescence shows Klf4; (d) representation of the merged images (scale bar: 100 μm).
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