Induction of Fetal Hemoglobin In Vivo Mediated by a Synthetic γ-Globin Zinc Finger Activator
Figure 2
Human β-like globin gene expression in adult blood samples from gg1-VP64 β-YAC transgenic mice. Total RNA isolated from adult blood was subjected to qPCR analysis using SYBR Green. Primers for human γ- and β-globin were utilized, and the data was normalized to mouse α-globin or Gapdh gene expression. (a) γ-globin gene expression. (b) β-globin gene expression. Results are the average of 7 different gg1-VP64 β-YAC bigenic mice ± the standard error of the mean (SEM). Student’s t-test values were for γ-globin and P > 0.1 for β-globin.
