Anemia

Research Article

Detection of Asymptomatic Sickle Cell Hemoglobin Carriers and Fetal Hemoglobin Regulating Genetic Variants in African Descendants from Oaxaca, Mexico

Table 2

Primers’ sequences for detection of SNVs-HbF in BCL11A through ARMS-PCR.
SNV IDPrimerSequence 5′ ⟶ 3′
rs1427407FW 1AGT​TAG​GAC​TTC​CTT​TTA​CTG​TAC​T
RV 1ATAA​CCT​TCT​TAG​CAC​CCA​CAA​ACAT
RV 1BTAA​CCT​TCT​TAG​CAC​CCA​CAA​ACAC
rs7599488FW 2AAC​ACG​GAG​TGA​TGA​TGC​CTA​GGG​T
RV 2ACAA​AGA​AGT​TAG​TCT​CAG​CCA​CCT​GA
RV 2BCAA​AGA​AGT​TAG​TCT​CAG​CCA​CCT​GG
rs766432FW 3ATGA​ATG​ACT​TTT​GTT​GTA​TGT​AAAG
FW 3BTGA​ATG​ACT​TTT​GTT​GTA​TGT​AAAT
RV 3ATA​CTG​ATG​AAT​AAG​ACT​GAG​TT
rs11886868FW 4GAG​GTT​TCT​ATT​CGG​AAT​AGG​A
RV 4AATC​GTC​TTT​TGT​GTT​TAA​TTT​CTTC
RV 4BATC​GTC​TTT​TGT​GTT​TAA​TTT​CTTA
rs4671393FW 5GGT​TTC​TTA​AGT​AAT​GTA​GGT​G
RV 5AGCT​GTG​GAC​AGC​AAA​GCT​TCA
RV 5BGCT​GTG​GAC​AGC​AAA​GCT​TCC
rs7557939FW 6ACAG​CAT​CAC​CCT​CTC​TCA​CTC​TTGC
FW 6BCAG​CAT​CAC​CCT​CTC​TCA​CTC​TTGT
RV 6GTT​GAC​CTC​CCC​CAT​TAG​CAG​CAT​G
Three primers were designed to identify the presence of each SNV: one primer with an SNV-free binding site paired with each primer to differentiate the base in the SNV. Additionally, one primer containing at the 3′ end one possible nucleotide of the SNV was evaluated, so the bold values in the primer column represent the alternative primers used to detect the SNVs, and the bold letters in the Sequence 5′ ⟶ 3′ column indicate the alternative nucleotides.