Effects of Menadione Sodium Bisulphite (Vitamin K<sub>3</sub>) Supplementation of the Diets Based on Plant Feed Ingredients on Growth and Bone Health of Gilthead Seabream (<i>Sparus aurata</i>) Fingerlings

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Gene</td><td class="align_center">Nucleotide sequence (5<span class="nowrap"><svg height="11.4859pt" id="M6" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>-</span>3<span class="nowrap"><svg height="11.4859pt" id="M7" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>)</span></td><td class="align_center">Accession number</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Beta-actin (<i>bact</i>)</td><td class="align_center">F: TCTGTCTGGATCGGAGGCTC</td><td class="align_center" rowspan="2">X89920</td></tr><tr><td class="align_center">R: AAGCATTTGCGGTGGACG</td></tr><tr><td class="align_left" rowspan="2">Elongation factor 1<i>α</i> (<i>ef1a</i>)</td><td class="align_center">F: CATGGTTGTGGAGCCCTTCT</td><td class="align_center" rowspan="2">AF184170</td></tr><tr><td class="align_center">R: TCCTGCACGACCATTCATTTC</td></tr><tr><td class="align_left" rowspan="2">Gla-rich protein (<i>grp</i>)</td><td class="align_center">F: CGCGATGAGTCAGATGAGAG</td><td class="align_center" rowspan="2">EU022752.1</td></tr><tr><td class="align_center">R: AAGGTGATGCTCCGAAGAGA</td></tr><tr><td class="align_left" rowspan="2">Alkaline phosphatase (<i>alp</i>)</td><td class="align_center">F: AGAACGCCCTGACGCTGCAA</td><td class="align_center" rowspan="2">AY266359</td></tr><tr><td class="align_center">R: TTCAGTATACGAGCAGCCGTCAC</td></tr><tr><td class="align_left" rowspan="2">Runt-related transcription factor 2 (<i>runx2</i>)</td><td class="align_center">F: GCCTGTCGCCTTTAAGGTGGTTGC</td><td class="align_center" rowspan="2">AJ619023</td></tr><tr><td class="align_center">R: TCGTCGTTGCCCGCCATAGCTG</td></tr><tr><td class="align_left" rowspan="2">Osteocalcin (<i>oc</i>)</td><td class="align_center">F: GGCAGCCATCTGTCTGACTT</td><td class="align_center" rowspan="2">AF048703</td></tr><tr><td class="align_center">R: GGTCCGTAGTAGGCCGTGTA</td></tr><tr><td class="align_left" rowspan="2">Bone morphogenic protein 2 (<i>bmp2</i>)</td><td class="align_center">F: GTGGCTTCCATCGTATCAACATTTT</td><td class="align_center" rowspan="2">JF261172.1</td></tr><tr><td class="align_center">R: GCTCCCCGCCATGAGT</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Sequences of primers used for gene expression analysis.</div>

Aquaculture Nutrition

tab2

Table 2

Table 2: Effects of Menadione Sodium Bisulphite (Vitamin K<sub>3</sub>) Supplementation of the Diets Based on Plant Feed Ingredients on Growth and Bone Health of Gilthead Seabream (<i>Sparus aurata</i>) Fingerlings 

Table 2 | Effects of Menadione Sodium Bisulphite (Vitamin K3) Supplementation of the Diets Based on Plant Feed Ingredients on Growth and Bone Health of Gilthead Seabream (Sparus aurata) Fingerlings 