Effects of Replacing Fishmeal with Defatted Black Soldier Fly (Hermetia illucens Linnaeus) Larvae Meal in Japanese Eel (Anguilla japonica) Diet on Growth Performance, Fillet Texture, Serum Biochemical Parameters, and Intestinal Histomorphology

Table 3

Proximate and amino acid composition of experimental diets.

	R0	R15	R30	R45	R60	R75
Proximate composition (g kg^−1)
Moisture	26.1	26.5	20.9	25.3	21.1	24.0
Crude protein	524.9	525.2	527.3	523.2	522.0	520.7
Crude lipid	83.6	83.3	83.7	84.0	83.4	87.3
Ash	128.1	125.4	118.5	113.9	108.4	102.9
Crude fiber	11.8	34.3	41.9	54.5	60.9	32.3
NFE	225.5	205.3	207.7	199.1	204.2	232.8
Gross energy (MJ kg^−1)	15.7	15.3	15.4	15.2	15.3	15.9
Amino acid (g kg^−1 crude protein)
Essential amino acid
Arginine	61.3	62.8	59.6	56.3	55.1	57.3
Histidine	22.8	24.9	25.1	25.6	27.0	28.7
Isoleucine	43.2	44.6	42.9	41.1	40.6	41.8
Leucine	78.8	80.8	77.3	73.4	72.5	73.5
Lysine	72.9	73.1	68.8	65.1	62.6	62.6
Methionine	17.3	18.5	17.8	14.8	13.9	13.0
Phenylalanine	42.9	44.3	42.9	41.0	41.0	42.4
Threonine	38.1	38.3	36.6	35.5	34.6	35.0
Tryptophan	1.1	1.2	1.0	1.5	1.7	1.6
Valine	49.7	52.5	51.9	51.7	52.5	54.7
Nonessential amino acid
Alanine	59.2	61.0	57.8	56.9	56.2	59.5
Aspartic acid	94.8	96.1	92.9	89.8	89.4	92.7
Cystine	7.3	7.3	6.5	6.2	6.3	6.3
Glutamic acid	147.2	146.3	138.6	131.2	126.3	133.6
Glycine	53.2	54.1	53.4	53.2	53.3	55.6
Proline	41.0	44.1	44.2	45.2	47.4	49.9
Serine	40.5	41.8	40.7	39.3	39.9	41.4
Tyrosine	35.8	41.1	43.0	46.4	50.8	55.1

Abbreviation: NFE: nitrogen-free extract.