Research Article
Changes in the Neuropeptide Y mRNA Expression in Oncorhynchus mykiss at Different Feeding Frequencies
Table 1
Experimental design for the changes in the neuropeptide Y mRNA expression in Oncorhynchus mykiss at different feeding frequencies.
| | Scheduled feeding duration (30 days) | Sampling on day 31 | | Treatments | Replicates | Feeding frequencies/feeding rate | Groups | Replicates | Sampling times | | 9:00 | 11:00 | 13:00 | 15:00 | 17:00 | 8:45 | 10:45 | 12:45 | 14:45 | 16:45 |
| | T1 | 6 | 5.0 %BW | × | × | × | × | T1 fed (feed based on previous days) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | | T1 unfed (starve) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | | T2 | 6 | 1.7 %BW | × | 1.7 %BW | × | 1.6 %BW | T2 fed (feed based on previous days) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | | T2 unfed (starve) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | | T3 | 6 | 1.0 %BW | 1.0 %BW | 1.0 %BW | 1.0 %BW | 1.0 %BW | T3 fed (feed based on previous days) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ | | T3 unfed (starve) | 3 | ✓ | ✓ | ✓ | ✓ | ✓ |
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