Evaluation of the Optimal Protein Required in Granulated Microdiets for Rockfish (Sebastes schlegeli) Larvae

Table 2

Feed ingredients of the experimental microdiets (%, dry matter basis).

	Experimental diets
	CP42	CP46	CP50	CP54	CP58	IV	LL	Crumble
Ingredients (%)
Sardine meal^a	16.00	18.00	20.00	22.00	24.00	Closed
Hydrolyzed fish soluble^b	16.00	18.00	20.00	22.00	24.00
Krill meal	27.00	29.00	31.00	33.00	35.00
Wheat gluten	2.00	2.00	2.00	2.00	2.00
Taurine	2.50	2.50	2.50	2.50	2.50
α-starch	3.00	3.00	3.00	3.00	2.00
Dextrin	21.00	15.50	10.00	4.50	0.00
Fish oil	6.00	5.50	5.00	4.50	4.00
Soybean lecithin	0.65	0.65	0.65	0.65	0.65
Vitamin premix^c	4.00	4.00	4.00	4.00	4.00
Mineral premix^d	1.00	1.00	1.00	1.00	1.00
Choline chloride (50%)	0.85	0.85	0.85	0.85	0.85
Nutrients (%)
Dry matter	98.2	98.3	98.7	99.1	98.5	95.6	98.2	97.4
Crude protein	42.4	46.5	50.3	54.2	58.2	56.0	57.1	56.0
Crude lipid	14.0	14.4	14.5	14.7	15.1	10.4	14.7	8.9
Ash	8.8	8.5	8.6	8.5	8.5	8.5	12.2	11.0
Gross energy (kJ/g diet) ^e	18.2	18.4	18.4	18.4	18.5	17.5	17.8	16.8
P/E (mg protein/kJ)	23.2	25.3	27.4	29.4	31.4	31.9	32.0	33.3

^aSardine meal was imported from Chile. ^bHydrolyzed fish soluble was imported from France. ^cVitamin premix contained the following amount which were diluted in brewer’s yeast (mg kg/diet): L-ascorbic acid, 51.24; DL-α-tocopheryl acetate, 150.0; thiamin hydrochloride, 20.0; riboflavin, 40.0; pyridoxine hydrochloride, 20.0; nicotinic acid, 150.0; D-calcium-pantothenate, 70.0; inositol, 300.0; D-biotin, 0.2; folic acid, 10.0; p-aminobenzoic acid, 18.2; menadione sodium hydrogen sulfite, 10.0; retinyl acetate, 6.0; cyanocobalamin, 0.001. ^dMineral premix contained the following amount which were diluted in brewer’s yeast (mg kg/diet): MgSO₄·7H₂O, 496.92; C₄H₂FeO₄, 65.8; FeSO₄, 103.04; CuSO₄, 5.97; CoSO₄.7H₂O, 3.42; CaI₂, 3.91; ZnSO₄, 68.85; Al (OH)₃, 3.81; MnSO₄·H₂O, 65.8. ^eGross energy is calculated based on 16.7 kJ/g for protein and carbohydrate and 37.7 kJ/g for lipid (Garling and Wilson, 1976).