Methionine-Mediated Regulation of Intestinal Structure and Lipid Transport in the Rice Field Eel (Monopterus albus)

Table 1

Composition of the diets and level of nutrition (g/kg).

Ingredients	M0	M2	M4	M6	M8	M10
Fish meal	110	110	110	110	110	110
Soy protein concentrate	400	400	400	400	400	400
Fish oil	40	40	40	40	40	40
DL-Methionine^1	0	2	4	6	8	10
Lysine	3.6	3.6	3.6	3.6	3.6	3.6
Glycine	16	14	12	10	8	6
Glutamate	4	4	4	4	4	4
Food attractant^2	1	1	1	1	1	1
Wheat meal	138.4	138.4	138.4	138.4	138.4	138.4
α-Starch	200	200	200	200	200	200
Brewer yeast	50	50	50	50	50	50
Choline chloride	5	5	5	5	5	5
Ca(H2PO4)2	20	20	20	20	20	20
Vitamin and mineral premix^3	12	12	12	12	12	12
Total	1000	1000	1000	1000	1000	1000
Proximate analysis
Dry matter (g/kg)	922.66	925.27	928.12	928.43	923.63	924.78
Crude protein (g/kg)	445.92	443.41	458.73	447.40	451.84	450.77
Crude lipid (g/kg)	67.86	67.11	68.69	67.70	67.92	68.07
Crude ash (g/kg)	102.60	101.90	100.60	102.60	101.90	100.60
Gross energy (kJ/g)	19.10	18.86	18.74	19.17	19.25	19.10

¹DL-Methionine (BR, 99%): Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). ²Attractants: 40% betaine, 20% DMPT, 20% threonine, 10% glycine, and 10% inosine-5-diphosphate trisodium salt. ³Vitamin and mineral premix: MGOTer Bio-Tech Co. Ltd (Qingdao, Shandong, China); premix composition (mg/kg diet): KCl, 200 mg; KI (1%), 60 mg; CoCl₂·6H₂O (1%), 50 mg; CuSO₄·5H₂O, 30 mg; FeSO₄·H₂O, 400 mg; ZnSO₄·H₂O, 400 mg; MnSO₄·H₂O, 150 mg; Na₂SeO₃·5H₂O (1%), 65 mg; MgSO₄·H₂O, 2000 mg; zeolite power, 3645.85 mg; VB₁, 12 mg; riboflavin, 12 mg; VB₆, 8 mg; VB₁₂, 0.05 mg; VK₃, 8 mg; inositol, 100 mg; pantothenic acid, 40 mg; niacin acid, 50 mg; folic acid, 5 mg; biotin, 0.8 mg; VA, 25 mg; VCP₁, 5 mg; VE, 50 mg; VC, 100 mg; ethoxyquin, 150 mg; wheat meal, 2434.15 mg.