Methionine-Mediated Regulation of Intestinal Structure and Lipid Transport in the Rice Field Eel (Monopterus albus)

Table 4

Primer sequence for q-PCR.

Gene	Forward (5-3)	Reverse (5-3)	Accession no.	Size (bp)
gcn2^1	GGAACTCGTCCTGAACTG	TGGTGAAGAACTTGCCTAT	XM_020586241.1	298
eif2a^2	CCCCTTCCTTTGTTCGTC	GCTGAGGCTTTCTTGTTCC	XM_020621840.1	121
lpl^3	CGTTGACATCGGAGACCTGA	CAAAGACCACCTTGGACTGAG	XM_020613041.1	146
moat2^4	TCTCCCTGCCTCTCTTTCA	TGTCCACTCCATAGTTGCCT	XM_020622089.1	213
dgat2^5	ACTTCCGCTTTCCCTTG	ATTCCCTGTCTCGTTATGTG	XM_020622054.1	104
mttp^6	AAGATGCTCCAGGCTTTGTT	TGTCAGGACCCTCTAAAATCAG	XM_020602163.1	172
hdlbp^7	CCACCCCAGACGACAAAGAC	GGCGAGCAACAAAATAACGA	XM_020609988.1	165
ldlrap^8	CAGGAAGACAAAAGCAAGAAGG	CGAGTGGGGTTACTATGAGGC	XM_020617284.1	194
vldlr^9	ACATCCGTCGTTTGGGTCTA	GTGGTAGTGTCCCCTCGTTT	XM_020601062.1	169
npc1l1^10	TTGGAGTCCCAGTTTATTT	TACACTTGCGTCCACATT	XM_020590431.1	297
mct1^11	TCCTATGCCTTCCCTAAAT	AAGTTGAATGCCAGTCCC	XM_020586598.1	287
mct2^12	TGGGCTTGTCACCATTAT	CTCCTCGTCCAGTTTCTT	XM_020589687.1	181
mct4^13	GAGGAGCAGTGGTGGATG	GGGAAGGCGTAGGAGAAA	XM_020608921.1	112
mct7^14	GTTGTCATTGGCACCCTT	ACCTGAGTCCTCCGAACC	XM_020608232.1	210
mct8^15	CAGCAGGACCTTCCAAAT	AAAGTAGCCCAGGACAGC	XM_020592011.1	271
mct12^16	GTTGGCGTATGGGATTGC	TTTGGCGAGATTTGGATGT	XM_020616669.1	223
cd36^17	TTGAAAGGGATTGAGGTG	TCTCGCAAGGATGGACTA	XM_020616796.1	212
fatp1^18	GCGAGCCAGGTATGTTAG	CAGCAAGGCACTGAGGAC	XM_020587461.1	263
fatp2^19	CTTTGATTACAGCCTTGC	CTTTCCGTTGTCCTTTCT	XM_020602138.1	100
fatp6^20	CAGTAGGACTTTGGGCATTT	GTCGCACTTTGTGAACTTTATC	XM_020618747.1	267
fatp7^21	ACTGTAATCATCAGCCAAGA	GGTTTCGTCAAACTCCTC	XM_020588511.1	105
apo^22	GGGCTGCTCTGGATGTCT	CCCGCAAAGCACTAATCT	HQ603782.1	147
apoa^23	CAAGAAGGTCCAGGTTGA	TTAGTAAGGGATTGGTAGAGG	XM_020590134.1	147
apob^24	TGCCAATAACTATCCGCTAC	TCTTCCTGACATCATCCC	XM_020615697.1	247
apoc^25	GCTGCTGGTCGTTACTGT	AGTCCCTAATGGTTTCTATG	XM_020590135.1	176
apoe^26	CGCTGCGTGGAAGGAAAC	CTGCCAGAGCAAGGATGAGA	XM_020590131.1	220
apof^27	AGGTGGTAAGCCTGATAGA	CCAACCCTCATAGTGTCC	XM_020592847.1	185
apoo^28	GCTCAGGTTCGGTTTGTT	GGTGGCAACTCTGGGTAT	XM_020595548.1	207
occ^29	TGTCGGGGAGTGGGTAAA	TCCAGGCAAATAAAGAGGCT	XM_020616177.1	130
zo-1^30	GGCATCATCCCCAACAAA	GCGAAGACCACGGAACCT	XM_020621576.1	111
zo-2^31	AGCCGAGGTCGCACTTTA	GCTTTGCTTCTGTGGTTGAT	XM_020615114.1	246
cl-12^32	TCACCTTCAATCGCAACG	ATGTCTGGCTCAGGCTTATCT	XM_020607277.1	250
cl-15^33	CTCGCTGCTTGCTTTGACT	TTGAAGGCGTACCAGGACA	XM_020611334.1	225
rpL17^34	CGAGAACCCGACTAAATCA	GTTGTAGCGACGGAAAGG	XM_020587712.1	169

¹gcn2: general control nonderepressible; ²eif2a: eukaryotic translation initiation factor 2; ³lpl: lipoprotein lipase; ⁴moat2: monoacylglycerol O-acyltransferase 2; ⁵dgat2: diacylglycerol acyltransferase 2; ⁶mttp: microsomal triglyceride transfer protein; ⁷hdlbp: high-density lipoprotein binding protein; ⁸ldlrap: low-density lipoprotein receptor adapter protein; ⁹vldlr: very low-density lipoprotein receptor; ¹⁰npc1l1: NPC1 like intracellular cholesterol transporter 1; ¹¹mct1: monocarboxylate transporter 1-like; ¹²mct2: monocarboxylate transporter 2-like; ¹³mct4: monocarboxylate transporter 4-like; ¹⁴mct7: monocarboxylate transporter 7-like; ¹⁵mct8: monocarboxylate transporter 8-like; ¹⁶mct12: monocarboxylate transporter 12-B-like; ¹⁷cd36: CD36 molecule; ¹⁸fatp1: fatty acid transport protein 1; ¹⁹fatp2: fatty acid binding protein 2; ²⁰fatp6: fatty acid transport protein 6; ²¹fatp7: fatty acid binding protein 7; ²²apo: apolipoprotein; ²³apoa: apolipoprotein A; ²⁴apob: apolipoprotein B; ²⁵apoc: apolipoprotein C; ²⁶apoe: apolipoprotein E. ²⁷apof: apolipoprotein F; ²⁸apoo: apolipoprotein O; ²⁹occ: occludin-like; ³⁰zo-1: tight junction protein ZO-1-like; ³¹zo-2: tight junction protein ZO-2-like; ³²cl-12: claudin 12; ³³cl-15: claudin 15; ³⁴rpL17: ribosomal protein L17; it is reference gene. NCBI Reference Sequence.