Fish Oil Replacement with Poultry Oil in the Diet of Tiger Puffer (Takifugu rubripes): Effects on Growth Performance, Body Composition, and Lipid Metabolism

Table 3

Sequence information of the primers used in this work.

Primer	Sequence (5-3)	GenBank reference	PL (bp)
Lipid metabolism genes
Lipogenesis
acacβ-F	GAAAGGTTTGCTGTGCGACTA	XM_011615767.1	154
acacβ-R	TTACATCAGCGACCATTTCAGT
fas-F	CTTTGCCGCTGTCATTCG	XM_011619859.1	78
fas-R	TGTCTCAACCCATTTGTAGTCG
β-Oxidation
cpt-1-F	GGGGTTTGTGGTCAAGTTAGG	XM_011607269.1	186
cpt-1-R	ATAGATCCGTGGCGCTCAT
vlc-qF	CGCTGTTCTTGGTGTTGGAC	XM_003969871.3	276
vlc-qR	GAGATTTGCTGCGGATGTTG
acox1-qF	GCACGGCATCGCAAGTAAC	XM_029850253.1	145
acox1-qR	GAGATCGAAGGCATCCACC
acox3-qF	GACTGTGGCTATCCGCTTCT	XM_029839734.1	214
acox3-qR	TTCCTGTCGGTCACTCTTGT
ehhadh-qF	GGCACAATGGGAAGAGGCATT	XM_003961946.3	185
ehhadh-qR	TGGACGGTTTCGCTGTAGGTA
acaa1-qF	GGACAACAGCAAAGCAAGAG	XM_029849183.1	110
acaa1-qR	ACCAGAAAAAACAGCCAAAA
acaa2-qF	ACGGGGGTGTTTTGAAGGA	XM_003975006.3	159
acaa2-qR	CATGACGGGCAATGTAGGG
gpat-F	CCCGTTCACAAATCCCACA	XM_011621885.1	235
gpat-R	GGCACAACAACTCCTCCGTAT
dgat1-F	TGGTTTGTGAGCCGTTTCC	XM_003969352.2	185
dgat1-R	CTGGCATTCGTTTGACTTCG
mgat2a-F	AAAGGCTTCATTAAATTGGC	XM_003978609.3	223
mgat2a-R	TGATGGCTTGTCTGTAGGG
Hydrolysis of glycerides
atgl-F	CCAACCTCTACAGGGTCTCA	XM_003967696.3	119
atgl-R	GTTTAGCAGCCCGTTCTTC
daglα-F	CTGTTGGTGGAGTTGGTGTATG	XM_011610175.1	72
daglα-R	ATCAGAGCACGGCTGGTAAT
hsl-F	CTCTTGCTATCGGTCTTGTGG	XM_011621066.1	113
hsl-R	TTCTGGGTCAATGGCATACTT
mgll-F	CCATCCAGTCAAAGTGGGTCT	XM_003963030.2	110
mgll-R	CATCAGCTGCATGCCGAA
Lipid digestion
bsal-F	TTGAAGATGACTGACCCCGA	XM_003978375.2	162
bsal-R	GATGTCTGCTGCGTTGTGAA
lp-F	CGTTTTCTCCTGTTCACCC	XM_029832009.1	97
lp-R	GACTCGTCCTCATCCCACT
Lipid transport
lpl-F	AGGGTCCACATCCGCAAA	NM_001305600.1	157
lpl-R	GTTTCTCCTTGCGGCTCAT
lipc-F	GCGGCTTCAACAGCAGTAA	XM_011610357.1	215
lipc-R	GAGGTGCGCTATGTCTTTCC
fabp1-F	CCATCGGTCTCCCTGATGAAG	XM_003974807.3	121
fabp1-R	TTGACCGTTACCTTCGGTCC
fabp10a-F	CTGTGACCAACTCCTTTACCAT	XM_003965635.3	150
fabp10a-R	TCTCTCCACCTTTGAGCTCCTG
fatp1-F	ATTGCAGACACCACAGGGAG	XM_003964742.3	219
fatp1-R	ATATCGTGACGCTCGTGCAT
apoa1-F	CGATGACGCCGAGTACAAA	AB183289.1	104
apoa1-R	CGGTTATGGGAGAAACGCTA
apoa4-F	TGCTTTCTGGGACTATGTTGC	NM_001078591.1	124
apoa4-R	GTTGACTTTGTCGGCACTCTC
apob100-F	AGGGACATAGTCAAACCAAGGA	XM_011619944.1	127
apob100-R	AGAACACGAAGGCTGGACAC
apoe1-F	TATTCAGACCCGCACCTCA	NM_001078592.1	201
apoe1-R	ATTTCCTCCATCTTGTCCTCC
mttp-F	ATGCTAAGGGTCTGGTTCTGC	XM_011612378.1	124
mttp-R	ATGTCAGTGCTGCCGATCTT
Lipid metabolism-related transcriptional factors
srebf1-F	TTTCAGCATCCCACCTTCC	XM_011603881.1	158
srebf1-R	GGTGAACCGTGAGGACAACTA
pparα1-F	TCAGTAGTTTATGGGTTGGTGG	NM_001097630.1	119
pparα1-R	GCGTGGACTCCGTAGTGGTA
pparα2-F	CCAGAAGAAGAACCGCAACA	NM_001097629.1	149
pparα2-R	CCTCTTTCTCCACCATCTTGTT
pparβ-F	AGCTGGAATACGACCGATGT	AB275887.1	249
pparβ-R	TCTTCAGGTAGGCGGAGTTG
pparγ-F	CGCTGTCCCGACATCTGTAT	NM_001097627.1	146
pparγ-R	GAACTGCTCGCCTTCCATT
fxr-F	GTGAACGACCACAAGTTTACCC	XM_003967283.2	166
fxr-R	AGACCAACAGATTACACCGGAT
lxrα-F	GTGACGCACCACTAACAGCA	XM_011609917.1	191
lxrα-R	CTGACAACACCGAGCAAGACT
hnf4α-F	GAGCCACGGGCAAACACTA	XM_011619034.1	199
hnf4α-R	AGGGTCCTACCTTCTTTCTTCAT
lrh-1-F	CGCTGACATGCTGCCTAAA	XM_003974281.2	140
lrh-1-R	TCTCGTCCAAGTCTTCGTCAT
Cholesterol and bile acid biosynthesis
hmgcr-F	GCTGCTGGCAATCAAGTACAT	XM_003974466.2	237
hmgcr-R	AAACATACAACTCCTTCCTACAGC
cyp7a1-F	CCTACCTGCTACCTTCTGGAGT	XM_003975521.2	143
cyp7a1-R	TCCTCTTTGGCAACACGAA
Reference gene
β-Actin-F	GAGAGGGAAATCGTGCGTGA	XM_003964421.3	186
β-Actin-R	GAAGGATGGCTGGAAGAGGG
ef1α-F	TTGGAGGCATTGGAACTGT	NM_001037873.1	86
ef1α-R	GTTGACGGGAGCAAAGGT
Mitochondrial DNA
16S rRNA-F	ATGTGGACCTGTATGAATGGC	NC_004299.1	119
16S rRNA-R	CTCCATAGGGTCTTCTCGTCTT
CYTB-F	CCTCCTGGGCTTCACAATCA	NC_004299.1	123
CYTB-R	TTAATGTGGGCGGGGGTAAC
β-Actin-F	GACGCAAAACCTCCGAACTG	Gene ID 101079312	129
β-Actin-R	CCTCCAAACGGATCAGCACA
EF1Α-F	TGGCCTTTAGCCGAATGAGG	Gene ID 653026	117
EF1Α-R	TGTCGGGCCAATCAATCCAG

acacβ: acetyl-CoA carboxylase beta; fas: fatty acid synthase; cpt-1: carnitine O-palmitoyltransferase-1; vlcs: very long-chain acyl-CoA synthetase; acox1: acyl-CoA oxidase 1, palmitoyl; acox3: acyl-CoA oxidase 3, pristanoyl; ehhadh: enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase; acaa: acetyl-CoA acyltransferase; gpat: glycerol-3-phosphate acyltransferase; dgat1: diacylglycerol O-acyltransferase 1; mgat2a: 2-acylglycerol O-acyltransferase 2-A-like (LOC101069338); atgl: adipose triglyceride lipase (patatin-like phospholipase domain containing 2 (pnpla2)); daglα: diacylglycerol lipase, alpha; hsl: hormone-sensitive lipase; mgll: monoglyceride lipase; bsal: bile acid activated lipase; lp: inactive pancreatic lipase-related protein 1-like (LOC101064949); lpl: lipoprotein lipase; lipc: lipase, hepatic; fabp: fatty acid binding protein; fatp: fatty acid transport protein (solute carrier family 27 member 1 (slc27a1)); apo: apolipoprotein; mttp: microsomal triglyceride transfer protein; srebf1: sterol regulatory element binding transcription factor 1; ppar: peroxisome proliferator-activated receptor; fxr: farnesoid X receptor (nuclear receptor subfamily 1, group H, member 4, NR1H4); lxrα: liver X receptor alpha (nuclear receptor subfamily 1, group H, member 3, NR1H3); hnf4α: hepatocyte nuclear factor 4, alpha; lrh-1: liver receptor homolog-1 (nuclear receptor subfamily 5, group A, member 2, NR5A2); hmgcr: 3-hydroxy-3-methylglutaryl-CoA reductase; cyp7a1: cholesterol 7-alpha-hydroxylase (cytochrome P450 family 7 subfamily A member 1); CYTB: cytochrome B; PL: product length.