Fish Meal Replacement by Mixed Plant Protein in the Diets for Juvenile Yellow Catfish Pelteobagrus fulvidraco: Effects on Growth Performance and Health Status

Table 2

Primers used for quantitative real-time PCR analysis.

Genes	Forward primer (5-3)	Reverse primer (5-3)	Accession no.
mTOR	GCCGATTTGCCAACTACCT	ACTCCAGAGCCCGCTTCAC	XM_027166728
s6	GCAAGCTCTTCAACCTGTCC	TTTCTCTTTGGCCTCCTTCA	XM_027144105
s6kb1	GTCCCGATGACTCCACACTT	ACTGGACTCACTGGCGTTCT	KY072932
eif4b	AACCACCCTCTCCAGGAAGT	CGTGGACAATCTCCCTTGTT	XM_027144909
4ebp1	GGGACTCTGTTTAGCACCAC	AAACTGGGCGTCTTCACC	XM_027140104
eif4e	TGACGAAGCCAGTGAAGATG	GTTTTTGACGGGGAGATTCA	XM_027142082
sod1	CCTCAAAGGCACAGGAGAAG	AATCGGCAGTCACATTACCC	XM_027171881
sod2	TCATGCAGCTTCACCATAGC	CTGTGGTTCTCCTCCACCAT	XM_027166181
gpx1	GATCAGACAGAGGCGTGACA	GCTGAGCTCTGCTGTACGTG	MN062284
Cat	CGTTTAGCCGCTACTGATCC	CTGGAATCAGGGGGTAGTCA	KX455919
nrf2	GTGAAGGGGGAAACACAGA	GCTCGTCCATGTCAGAGTCA	KX455917
keap1	CGAATCGCATTGCTTAGTCA	CGGAACTAAAAGGTGGTGGA	XM_027133478
il1β	CTGTGTGTTTGGGGATTGTG	GGGTATTTCACCGACTCGAA	MF770571
il6	ATGCCTCACCTAGAGCAGGA	GTGAAGCTGTGCAGAATGGA	XM_027176013
il8	GACTGCGATGCTTTGTGAAG	TCAGGCAGACCTTCATTCCT	KY218792
tnfα	TCTGCTTCACCATCTTCGTG	GGCACCAGCTTCTTGACTTC	XM_027165847
tnfβ	CGGTGGTCATCTCACACATC	CAGCTCAAACACCCCAAAAT	XM_027146388
il10	TCCTGCTTTCTTTGCTGACA	GAATAGTGCGGTGTCCAGGT	KY218793
tgfβ	ACAGGTCCAAAGGTTGATGG	CACATCCACAAGAAGCTGGA	XM_027154377
β-Actin	GGACTCTGGTGATGGTGTGA	CTGTAGCCTCTCTCGGTCAG	EU161066
rpl7	GGCAAATGTACAGGAGCGAG	GCCTTGTTGAGCTTGACGAA	KP938522
Hprt	ATGCTTCTGACCTGGAACGT	TTGCGGTTCAGTGCTTTGAT	KP938523
Tuba	TCAAAGCTGGAGTTCTCGGT	AATGGCCTCGTTATCCACCA	KP938526
b2m	GCTGATCTGCCATGTGAGTG	TGTCTGACACTGCAGCTGTA	KP938520
Ubce	TCAAGAAGAGCCAGTGGAGG	TAGGGGTAGTCGATGGGGAA	KP938524
Tbp	AGCAAAGAGTGAGGAGCAGT	ACTGCTGATGGGTGAGAACA	KP938525
Gapdh	TTTCAGCGAGAGAGACCCAG	ATGACTCTCTTGGCACCTCC	KP938521
18 s rRNA	AGCTCGTAGTTGGATCTCGG	CGGGTATTCAGGCGAGTTTG	KP938527
Elfa	GTCTGGAGATGCTGCCATTG	AGCCTTCTTCTCAACGCTCT	KU886307

Abbreviations: mtor: mammalian/mechanistic target of rapamycin; s6: ribosomal protein S6; s6kb1: ribosomal protein S6 kinase beta 1; eif4b: eukaryotic translation initiation factor 4B; eif4e: eukaryotic translation initiation factor 4E; 4ebp1: eukaryotic translation initiation factor 4E binding protein 1; sod1: superoxide dismutase 1; sod2: superoxide dismutase 2; gpx1: glutathione peroxidase 1; cat: catalase; nrf2: nuclear factor erythroid 2-related factor 2; keap1: kelch like ECH associated protein 1; il1β: interleukin 1 beta; il6: interleukin 6; il8: interleukin 8; tnfα: TNF alpha induced protein; tnfβ: tumor necrosis factor b; il10: interleukin 10; tgfβ: TGFB induced factor homeobox 1; elfa: translation elongation factor; gapdh: glyceraldehyde-3-phosphate dehydrogenase; hprt: hypoxanthine-guanine phosphoribosyl transferase; rpl7: ribosomal protein L7; tbp: TATA-box-binding protein; tuba: tubulin alpha chain; ubce: ubiquitin-conjugating enzyme.