Research Article
Lipotoxic Effects of Gossypol Acetate Supplementation on Hepatopancreas Fat Accumulation and Fatty Acid Profile in Cyprinus carpio
Table 1
Specific primers used in the real-time PCR amplification.
| | Metabolism function | Gene name | Primer(5-3) | Length/bp | Gene ID (accession number) |
| | Transcription factor | PPARα | F GCATGAAGCCTACCTCAGACACTT | 231 | FJ849065.1 | | R ACCGAGGCGTACTGGCAGAA | | PPARβ | F GCAGCACAGCCAGTCCTGAA | 128 | XM_019110518.1 | | R AACGCCGTAGTGGAATCCTGAC | | PPARγ | F GCAAGGCAGTGGAGGACAAGAAC | 121 | LOC109050306 | | R ACGCAACACAGCACCATAAGAGG | | SREBP-1 | F CACGGCTCTGCTCAACGACAT | 294 | XM_019073316.1 | | R TGCGGAGGAGACTGCTGGAA | | SREBP-2 | F CAGATGAGTGTGAGTCCGCAGAAG | 174 | XM_019064582.1 | | R CCATAGGCTGGTCCTCAGAGTCA | | Lipogenesis | FASN | F TCTGTGCTGTGCGGACTGGAA | 253 | KY378913.1 | | R GCAACATCGGCTGGATATTGAGGAG | | ACACβ | F ACTGGCTGGCTAGATCACCTTATTG | 207 | XM_019106587.1 | | R GCACCGCATACCACACCTAACAT | | Lipolysis | ATGL | F CACCAACACCTCCATTCAGTTCACA | 227 | KY906167.1 | | R ACTCTTCATCCTCCTCACCGTCAG | | HSL | F TTGATGCCTATGCTGGTACGAGTTG | 182 | MF061228.2 | | R TGATGTGGTTGGAGAGGATGATGCT | | PUFA biosynthesis | FADS2a | F CTGTAACATTGAGCAATCCGCCTTC | 236 | MK852165.1 | | R GGTACGCATCCAGCCAGAGTTC | | FADS2b | F AAGTAAGCAGCAGTCAGTCAGAGTT | 242 | MK852166.1 | | R CCGTGGCATCTTCTCCAGCATAG | | Lipid transport | CD36 | F CCGTAGGCACAGAGGAGGACATAT | 116 | KM030422.1 | | R GAGTGTGGAATTGGAGCGTTGGA | | FABP1b | F ATCGAATCCCTGACCGGAGA | 156 | XM_019124255.1 | | R CCTCTTGTAGACGAGGCTGC | | FABP10a | F AACCCCTGGAAAAACCGTCA | 176 | EU363800.1 | | R GGGTCTCCACCATCTCTCCT | | FABP10b | F AACTCCTTCACCATCGGCAA | 166 | EU363801.1 | | R CTCCTACCGTCAGGGTCTCC | | FABP11 | F TTGAGCTGTTGCTGGAGTGAC | 235 | XM_019119618.1 | | R GTTTTGAAGGTGCTCTGCGA | | Control | 18S | F CTGAGAAACGGCTACCACATC | 101 | FJ710827.1 | | R CAGACCTGTTATTGCTCCATCT |
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